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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Article DOI: 10.3791/50433-v • 11:00 min • May 20th, 2013
May 20th, 2013 •
1, 1, 1, 1, 1
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology, University of Pennsylvania

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Untargeted metabolomics provides a hypothesis generating snapshot of a metabolic profile. This protocol will demonstrate the extraction and analysis of metabolites from cells, serum, or tissue. A range of metabolites are surveyed using liquid-liquid phase extraction, microflow ultraperformance liquid chromatography/high-resolution mass spectrometry (UPLC-HRMS) coupled to differential analysis software.

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Untargeted Metabolomics Biological Sources Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS) Metabolic Profiles Cells Serum Tissue Liquid-liquid Phase Extraction Downstream Analysis Polar Metabolites Non-polar Metabolites Liquid Chromatography Methods Microflow Ultra-performance (UP)LC Methods Mass Spectrometer General Source Conditions Compound Optimized Source Conditions Full Scan Mode Positive Mode Negative Mode M/z Range High Resolution Calibrated Instrument Label-free Differential Analysis Bioinformatics Platforms Metabolic Pathway Screening Biomarker Discovery Drug Development
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