Journal
/
Chemistry
/
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

DOI:
10.3791/50433-v

•

11:00 min

•

May 20, 2013

•

, , , ,
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology,University of Pennsylvania

Chapters

  • 00:05Title
  • 01:20Sample Extraction from Cells
  • 02:34Re-suspension and Filtration of Samples for UPLC
  • 03:49UPLC and Mass Spectrometer Setup
  • 06:37Differential Analysis
  • 08:47Results: Analysis of Metabolites Using UPLC-HRMS
  • 10:42Conclusion

Summary

Automatic Translation

Automatic Translation

Untargeted metabolomics provides a hypothesis generating snapshot of a metabolic profile. This protocol will demonstrate the extraction and analysis of metabolites from cells, serum, or tissue. A range of metabolites are surveyed using liquid-liquid phase extraction, microflow ultraperformance liquid chromatography/high-resolution mass spectrometry (UPLC-HRMS) coupled to differential analysis software.

Tags

Keywords: Untargeted MetabolomicsUPLC-HRMSLiquid-liquid ExtractionPolar And Non-polar MetabolitesMicroflow UPLCPositive And Negative IonizationLabel-free Differential AnalysisMetabolic Pathway ScreeningBiomarker DiscoveryDrug Development

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50433?article_type=v

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
check_url/50433?article_type=v

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
check_url/50433?article_type=v

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
check_url/50433?article_type=v

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
check_url/50433?article_type=v

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
check_url/50433?article_type=v

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
check_url/50433?article_type=v

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos
check_url/50433?article_type=v

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
check_url/50433?article_type=v

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
check_url/50433?article_type=v

Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry

Read Article