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Chapters
- 00:05Title
- 01:22Culture and Expansion of Cytokine Transduced Stromal Cells
- 03:05Phospho-flow Cytometry Analysis of Stroma-produced Cytokine Activity
- 05:09Injection of Cytokine-transduced Stroma into Mice
- 06:22Serial Blood Collection and Plasma Monitoring of Exogenous Human Cytokine in Mice
- 08:33Human Hematopoietic Cell Transplantation
- 09:53Results: Achieving Physiological Levels of the Human TSLP Cytokine in PDX Mice
- 11:22Conclusion
Beskrevet her er en fremgangsmåde til fremstilling af eksogent cytokin i patient-afledte xenograftmus (PDX) via ugentlig intraperitoneal injektion af en cytokin-transduceret stromalcellelinie. Denne metode udvider anvendeligheden af PDX og giver mulighed for transient eller vedvarende eksogen cytokinlevering i en lang række PDX-modeller.
