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1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT),Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology,National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology,Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Chapters
- 00:05Title
- 01:35Nucleic Acid Extraction
- 04:27Validation of rDNA Based Primers with DNA Templates
- 06:18gDNA Contamination Assay with RNA Templates
- 07:49RT-PCR Step for cDNA Synthesis and qPCR Analysis
- 09:55Results: Use of rDNA Based Primers to Detect Genomic DNA in RNA Samples of LeadTissues
- 12:23Conclusion
Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific for the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) genes. The method is suited for reliable and sensitive detection of DNA contamination in most eukaryotes and prokaryotes.
