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基于核糖体 dna 的 RNA 样本 dna 污染评价
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
DOI:

13:16 min

January 22, 2018

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Chapters

  • 00:05Title
  • 01:35Nucleic Acid Extraction
  • 04:27Validation of rDNA Based Primers with DNA Templates
  • 06:18gDNA Contamination Assay with RNA Templates
  • 07:49RT-PCR Step for cDNA Synthesis and qPCR Analysis
  • 09:55Results: Use of rDNA Based Primers to Detect Genomic DNA in RNA Samples of LeadTissues
  • 12:23Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个在 RNA 样本中追踪基因组 DNA (gDNA) 污染的协议。所提出的方法利用特定的引物的内部转录间隔区 (其) 的核糖体 DNA (rDNA) 基因。该方法适用于大多数真核生物和原中 DNA 污染的可靠和灵敏检测。

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