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1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT),Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology,National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology,Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Chapters
- 00:05Title
- 01:35Nucleic Acid Extraction
- 04:27Validation of rDNA Based Primers with DNA Templates
- 06:18gDNA Contamination Assay with RNA Templates
- 07:49RT-PCR Step for cDNA Synthesis and qPCR Analysis
- 09:55Results: Use of rDNA Based Primers to Detect Genomic DNA in RNA Samples of LeadTissues
- 12:23Conclusion
Här presenterar vi ett protokoll för att spåra genomisk DNA (gDNA) kontaminering i RNA-prover. Den presenterade metoden använder tredjeparts primers som är specifika för regionen inre transkriberas spacer (ITS) ribosomal DNA (rDNA) gener. Metoden passar för tillförlitlig och känslig detektion av DNA kontaminering i de flesta Eukaryoter och prokaryoter.
