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Journal / Biology / Utnytte pHluorin-merket reseptorer å overvåke…
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Utnytte pHluorin-merket reseptorer å overvåke subcellulære lokalisering og menneskehandel
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Utnytte pHluorin-merket reseptorer å overvåke subcellulære lokalisering og menneskehandel

Article DOI: 10.3791/55466 • 09:59 min
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

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March 16th, 2017

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Merking av ekstracellulære domenet til en membran protein med en pH-sensitive fluorofor, superecliptic pHluorin (SEP), kan subcellulære lokalisering, uttrykk og menneskehandel skal fastsettes. Imaging september-merkede proteiner med total indre refleksjon fluorescens mikroskopi (TIRFM) muliggjør kvantifisering av proteinnivåer i den perifere ER og plasmamembran.

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