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Journal / Biology / 利用pHluorin标记的受体监视亚细胞定位和贩运…
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利用pHluorin标记的受体监视亚细胞定位和贩运

Article DOI: 10.3791/55466 • 09:59 min
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

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March 16th, 2017

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标签膜蛋白的胞外结构域与pH敏感荧光，superecliptic pHluorin（SEP），允许确定的亚细胞定位，表达和贩卖。成像SEP-标记的蛋白质与全内反射荧光显微镜（TIRFM）使蛋白质水平在外围ER和质膜的量化。

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