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CAPRRESI: Chimera Assembly genom Plasmid Recovery och Restriction Enzym Site Insertion

Article DOI: 10.3791/55526-v • 07:37 min • June 25th, 2017
June 25th, 2017 •
1, 2, 3
1Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, 2Oncomedic Incorporation, Ciudad de México, 3Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México

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Här presenterar vi chimärmontering genom plasmidåtervinning och restriktionsenzymställeinsättning (CAPRRESI), ett protokoll baserat på införandet av restriktionsenzymställen i synonym-DNA-sekvenser och funktionell plasmidåtervinning. Detta protokoll är en snabb och billig metod för att fusera proteinkodande gener.

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Molecular Biology Chimeric Protein Encoding Gen Assembly Synonym DNA Sequence Insertion Insertion of Restriction Enzyme Site Plasmid Recovery PUC18 / 19 Vector Disruptions of Antibiotics Resistance Gene Genetics Fusion Protein Primary Sigma Factor RpoD SigA
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