Journal
/
Biology
/
CAPRRESI: Chimera Assembly ved Plasmid Recovery og Restriction Enzyme Site Insertion
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly ved Plasmid Recovery og Restriction Enzyme Site Insertion

DOI:
10.3791/55526-v

•

07:37 min

•

June 25, 2017

•

, ,
1Centro de Ciencias Genómicas,Universidad Nacional Autónoma de México, 2Oncomedic Incorporation, Ciudad de México, 3Laboratorio Internacional de Investigación sobre el Genoma Humano,Universidad Nacional Autónoma de México

Chapters

  • 00:05Title
  • 01:22CAPRRESI Oligonucleotide Design
  • 03:29Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
  • 05:15Plasmid Recovery Using DNA Ligation and Bacterial Transformation
  • 06:43Results: Example Sequence of Assembled Gene
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi chimera-samling ved plasmidgenvinding og restriktionsenzym site insertion (CAPRRESI), en protokol baseret på indsættelsen af ​​restriktionsenzymsteder i synonym-DNA-sekvenser og funktionel plasmidgenvinding. Denne protokol er en hurtig og billig metode til fusion af proteinkodende gener.

Tags

CAPRRESIChimera AssemblyPlasmid RecoveryRestriction Enzyme Site InsertionGene FusionMolecular BiologyRpoDSigAAmpicillin ResistancePCRPerl ScriptsAmino Acid Sequence AlignmentSynonym DNARestriction Enzyme Sites

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Principles of Site-Specific Recombinase (SSR) Technology

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

High-throughput Yeast Plasmid Overexpression Screen

Overvågning plasmidreplikation Live pattedvrceller over flere generationer ved fluorescensmikroskopi

Generation af Plasmidvektorer udtrykker FLAG-mærkede proteiner henhold til forordningen for Human elongeringsfaktor-1α Promoter Brug Gibson Assembly

Fækal Glukokortikoid Analyse: Non-invasive Adrenal Overvågning i dyr fra hestefamilien

Multi-enzym Screening Ved hjælp af en High-throughput Genetic Enzyme Screening System

Read Article