JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
CAPRRESI:通过质粒恢复和限制酶位点插入的嵌合体装配
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
Please note that all translations are automatically generated.
Click here for the English version.
CAPRRESI:通过质粒恢复和限制酶位点插入的嵌合体装配
DOI:
10.3791/55526-v
•
07:37 min
•
June 25, 2017
•
Orlando Santillán
,
Miguel A. Ramírez-Romero
,
Guillermo Dávila
1
Centro de Ciencias Genómicas
,
Universidad Nacional Autónoma de México
,
2
Oncomedic Incorporation, Ciudad de México
,
3
Laboratorio Internacional de Investigación sobre el Genoma Humano
,
Universidad Nacional Autónoma de México
Chapters
00:05
Title
01:22
CAPRRESI Oligonucleotide Design
03:29
Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
05:15
Plasmid Recovery Using DNA Ligation and Bacterial Transformation
06:43
Results: Example Sequence of Assembled Gene
07:18
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们通过质粒恢复和限制性酶切位点插入(CAPRRESI)提供嵌合体装配,这是基于将限制酶位点插入同义词DNA序列和功能质粒恢复的方案。该协议是一种快速和低成本的融合蛋白质编码基因的方法。
Tags
CAPRRESI
Chimera Assembly
Plasmid Recovery
Restriction Enzyme Site Insertion
Gene Fusion
Molecular Biology
RpoD
SigA
Ampicillin Resistance
PCR
Perl Scripts
Amino Acid Sequence Alignment
Synonym DNA
Restriction Enzyme Sites
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
特定地点的重组酶(SSR)技术的原则
大会,加载和分析超速离心机样品池对齐
原代培养及质粒电穿孔尔蒂小鼠器官。
生产转基因
非洲爪蟾
限制性内切酶介导的整合和核移植
实心板为基础的饮食限制在
线虫</em
高通量酵母质粒过表达屏幕
在Live哺乳动物细胞中质粒复制多代荧光显微镜监测
质粒载体产生表达FLAG标记的蛋白质在人延伸因子1α启动子的使用吉布森大会规则
糖皮质激素粪分析:在马属动物无创监测肾上腺
多酶筛选使用高通量基因酶的筛选系统
Read Article