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CAPRRESI：通过质粒恢复和限制酶位点插入的嵌合体装配
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JoVE Journal Biology
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
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CAPRRESI：通过质粒恢复和限制酶位点插入的嵌合体装配

DOI:
10.3791/55526-v

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07:37 min

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June 25, 2017

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, ,
1Centro de Ciencias Genómicas,Universidad Nacional Autónoma de México, 2Oncomedic Incorporation, Ciudad de México, 3Laboratorio Internacional de Investigación sobre el Genoma Humano,Universidad Nacional Autónoma de México

Chapters

  • 00:05Title
  • 01:22CAPRRESI Oligonucleotide Design
  • 03:29Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
  • 05:15Plasmid Recovery Using DNA Ligation and Bacterial Transformation
  • 06:43Results: Example Sequence of Assembled Gene
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们通过质粒恢复和限制性酶切位点插入（CAPRRESI）提供嵌合体装配，这是基于将限制酶位点插入同义词DNA序列和功能质粒恢复的方案。该协议是一种快速和低成本的融合蛋白质编码基因的方法。

Tags

Keywords: CAPRRESIChimera AssemblyPlasmid RecoveryRestriction Enzyme Site InsertionGene FusionMolecular BiologyRpoDSigAAmpicillin ResistancePCRPerl ScriptsAmino Acid Sequence AlignmentSynonym DNARestriction Enzyme Sites

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