JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
S100A12的表达,纯化和抗菌活性
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Expression, Purification, and Antimicrobial Activity of S100A12
Please note that all translations are automatically generated.
Click here for the English version.
S100A12的表达,纯化和抗菌活性
DOI:
10.3791/55557-v
•
11:10 min
•
May 13, 2017
•
Emmanuel Jackson
,
Saffron Little
,
Dana S. Franklin
,
Jennifer A. Gaddy
3
,
Steven M. Damo
4
1
Department of Life and Physical Sciences
,
Fisk University
,
2
Tennessee Valley Healthcare Systems
,
U. S. Dept. of Veterans Affairs
,
3
Department of Medicine – Division of Infectious Diseases
,
Vanderbilt University Medical School
,
4
Department of Biochemistry
,
Vanderbilt University
Chapters
00:05
Title
01:17
Expression of S100A12
03:20
Purification of S100A12 Using Low Pressure Chromatography
06:27
Antimicrobial Activity Assays
07:58
Results: The Expression, Purification, and Antimicrobial Activity of S100A12
09:42
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们提出了一种表达和纯化S100A12(钙粒蛋白C)的方法。我们描述了一种测定其对人类
幽门螺杆菌的抗
微生物活性的
方案
。
Tags
S100A12
Antimicrobial Peptide
Protein Expression
Protein Purification
Antimicrobial Activity
Metal Sequestration
Oligomerization
Calprotectin
BL21 DE3 Cells
Autoinduction Media
Centrifugation
Sonication
Affinity Chromatography
Size Exclusion Chromatography
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
热稳定,表达,纯化,和人类血清素转运蛋白结晶绑定到<em>取值</em> -citalopram
光谱分析 TRPV1 的纯化与重构
哺乳动物 Bestrophin 离子通道的表达与纯化
植物细胞悬浮液中咖啡因的提取、酶活性及咖啡因合成酶基因的表达
氧电极对 DesB 酶活性和抑制作用的厌氧蛋白纯化及动力学分析
单粒子冷冻电子显微镜在结构中测定脂质敏感分通道 trpc3 的表达与纯化
真核硼酸背液转运体的表达、增溶及纯化
无核酸酶的表达和纯化原氧基质 3,4-二氧酶
富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
PCR 突变体、克隆、表达、快速蛋白质纯化协议和色氨酸合成酶野生类型和突变形式的结晶
Read Article