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JoVE Journal
Biochemistry

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
 

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Article doi: 10.3791/57796
July 3rd, 2018

Summary July 3rd, 2018

This article describes specific methods to obtain biochemical quantities of detergent-solubilized TRPV1 for spectroscopic analysis. The combined protocols provide biochemical and biophysical tools that can be adapted to facilitate structural and functional studies for mammalian ion channels in a membrane-controlled environment.

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