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Developmental Biology
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斑马鱼急性酒精性肝损伤的组织学分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼急性酒精性肝损伤的组织学分析
DOI:
10.3791/55630-v
•
10:45 min
•
May 25, 2017
•
Jillian L. Ellis
,
Chunyue Yin
2
1
Division of Gastroenterology, Hepatology and Nutrition
,
Cincinnati Children’s Hospital Medical Center
,
2
Division of Developmental Biology
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:05
Title
00:50
Acute Ethanol Treatment in Zebrafish Larvae
01:57
Preparation of Tissue Cassettes and Processing
04:02
Tissue Sectioning
05:49
Hematoxylin and Eosin (H&E) Staining of Paraffin-embedded Sections
08:44
Results: Comparisons of H&E Staining in the Livers of Zebrafish Larvae
10:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案描述了用2%乙醇处理24小时的斑马鱼幼虫肝脏的组织学分析。这种急性乙醇治疗导致肝血管的肝脂肪变性和肿胀。
Tags
Zebrafish
Histology
Acute Alcoholic Liver Injury
Patho-histology
Ethanol
Alcohol Liver Disease
2% Ethanol
Dietrich’s Fixative
PBS
3% Agarose
Sagittal Sections
Article
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