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Histologiske analyser af akut alkoholisk leverskader i sebrafisk
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
DOI:

10:45 min

May 25, 2017

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Chapters

  • 00:05Title
  • 00:50Acute Ethanol Treatment in Zebrafish Larvae
  • 01:57Preparation of Tissue Cassettes and Processing
  • 04:02Tissue Sectioning
  • 05:49Hematoxylin and Eosin (H&E) Staining of Paraffin-embedded Sections
  • 08:44Results: Comparisons of H&E Staining in the Livers of Zebrafish Larvae
  • 10:14Conclusion

Summary

Automatic Translation

Denne protokol beskriver histologiske analyser af leverne fra zebrafisklarver, som er blevet behandlet med 2% ethanol i 24 timer. En sådan akut etanolbehandling resulterer i hepatisk steatosis og hævelse af den hepatiske vaskulatur.

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