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Journal
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Biochemistry
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蛋白质tRNA琼脂糖凝胶延迟测定分析<em>ñ</em<sup> 6</sup> - 磺酰氨基甲酰腺苷TcdA功能
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Biochemistry
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JoVE Journal
Biochemistry
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the
N
6
-threonylcarbamoyladenosine TcdA Function
Please note that all translations are automatically generated.
Click here for the English version.
蛋白质tRNA琼脂糖凝胶延迟测定分析<em>ñ</em<sup> 6</sup> - 磺酰氨基甲酰腺苷TcdA功能
DOI:
10.3791/55638-v
•
08:03 min
•
June 21, 2017
•
Francisco J. Fernández
2,3
,
Sara Gómez
,
Sergio Navas-Yuste
,
Miguel López-Estepa
,
M. Cristina Vega
1
Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC)
,
Spanish National Research Council (CSIC)
,
2
Department of Immunology
,
Complutense University School of Medicine
,
3
Abvance Biotech srl
Chapters
00:05
Title
01:13
Preparation of the tRNA
02:44
Lysis, Extraction, and Purification of tRNA(UUU)
04:05
Sample Preparation
05:09
Gel Electrophoresis and Staining to Observe tRNA-Protein Interaction
06:33
Results: Evaluation of the Interaction Between TcdA and tRNA(UUU)
07:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
介绍了通过琼脂糖凝胶阻滞测定技术生产tRNA(UUU)和tDNA(UUU)与酶TcdA复合物的分析方案。
Tags
Protein-tRNA Interaction
Agarose Gel Retardation Assay
RNA-binding Proteins
TcdA Function
RNA Biology
Protein-RNA Complexes
E. Coli BL21 DE3
PET-23D EC Triple-U
IPTG Induction
Cell Lysis
Acid Phenol Extraction
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