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Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
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Biochemistry
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JoVE Journal Biochemistry
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

DOI:
10.3791/58369-v

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08:12 min

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September 28, 2018

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, ,
1Molecular Plant Biology, Department of Biochemistry,University of Turku

Chapters

  • 00:04Title
  • 00:35Preparing the Blue Native (BN) Gel
  • 03:09Thylakoid Solubilization
  • 04:19BN-PAGE
  • 05:052D-SDS-PAGE
  • 06:18Results: Protein Complex Pattern and the Composition of Thylakoid Protein Complexes Solubilized with β-DM and Digitonin
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.

Tags

Keywords: Thylakoid MembraneProtein ComplexesBlue Native Gel ElectrophoresisPhotosynthetic Protein ComplexesProtein Complex OrganizationGradient GelAcrylamide SolutionsPolymerizationStacking Gel

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