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Immunology and Infection
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Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages
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Immunology and Infection
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JoVE Journal Immunology and Infection
Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages

Phenotypic Analysis of Rodent Malaria Parasite Asexual and Sexual Blood Stages and Mosquito Stages

DOI:
10.3791/55688-v

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08:23 min

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May 30, 2019

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, , , , ,
1Beykoz Institute of Life Sciences and Biotechnology,Bezmialem Vakif University, Istanbul, Turkey, 2School of Public Health and Tropical Medicine, Department of Tropical Medicine,Tulane University

Chapters

  • 00:04Title
  • 00:47Frozen Parasite Stock Injection, Parasitemia Assessment, and Recipient Mouse Injection
  • 03:12Mosquito Infection and Oocyst Sporozoites Quantification
  • 05:07Salivary Gland Sporozoites Quantification
  • 06:22Results: Representative Rodent Malaria Parasite Infection Analyses
  • 07:54Conclusion

Summary

Automatic Translation

Automatic Translation

Due to the striking similarities of the life cycle and biology of rodent malaria parasites to human malaria parasites, rodent malaria models have become indispensable for malaria research. Herein, we standardized some of the most important techniques used in the phenotypic analysis of wild-type and transgenic rodent malaria species.

Tags

Rodent MalariaPhenotypic AnalysisAsexual Blood StageSexual Blood StageMosquito StageCryo Preserved Parasite StocksInfected ErythrocytesParasitemiaHeart PunctureBlood CollectionRecipient Mice

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