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Journal / Cancer Research / Ex Vivo Beeldvorming van Resident CD8 T…
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Cancer Research

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JoVE Journal Cancer Research
Ex Vivo Beeldvorming van Resident CD8 T lymfocyten in menselijke longen Tumor plakjes met behulp van confocale microscopie
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Ex Vivo Beeldvorming van Resident CD8 T lymfocyten in menselijke longen Tumor plakjes met behulp van confocale microscopie

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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Dit protocol beschrijft een methode om te afbeelding resident tumor-infiltreren CD8 T cellen aangeduid met fluorescently gekoppelde antilichamen binnen de menselijke longen tumor segmenten. Deze techniek staat real-time analyses van CD8 T-cel migratie met behulp van de confocal microscopie.

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Kankeronderzoek kwestie 130 tumorimmunologie mens Long tumor organotypic segmenten T-cel migratie fluorescentie microscopie confocal
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