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JoVE Journal Cancer Research
Paramyxovirussen voor Tumor-gerichte immunomodulatie
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Paramyxovirussen voor Tumor-gerichte immunomodulatie: ontwerp en evaluatie Ex Vivo

Article DOI: 10.3791/58651-v • 12:42 min • January 7th, 2019
January 7th, 2019 •
1,2, 1,3
1Department of Translational Oncology, German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT), 2Faculty of Biosciences, Heidelberg University, 3Department of Medical Oncology, NCT and Heidelberg University Hospital

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Dit protocol beschrijft een gedetailleerde workflow voor de generatie en ex vivo karakterisering van oncolytische virussen voor expressie van immunomodulators, met behulp van mazelen virussen bispecifieke T cel engagers codering als een voorbeeld. Toepassing en aanpassing aan andere vector platforms en transgenen zal de ontwikkeling van nieuwe immunovirotherapeutics voor klinische vertaling versnellen.

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Kankeronderzoek kwestie 143 oncolytische een kanker immunotherapie virale vectoren mazelenvirus paramyxovirus bispecifieke T-cel-engager
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