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Cancer Research
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Ex 活体
人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Ex Vivo
Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
Ex 活体
人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像
DOI:
10.3791/55709-v
•
08:28 min
•
December 27, 2017
•
Elisa Peranzoni*
1
,
Houcine Bougherara*
1
,
Sarah Barrin*
1
,
Audrey Mansuet-Lupo
3
,
Marco Alifano
,
Diane Damotte
3
,
Emmanuel Donnadieu
1
Institut Cochin, Inserm U1016-CNRS UMR8104
,
Université Paris Descartes
,
2
Department of Pathology, Paris Centre University Hospitals
,
Université Paris Descartes
,
3
INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center
,
University Pierre and Marie Curie
,
4
Department of Thoracic Surgery, Paris Centre University Hospitals
,
University Paris Descartes
Chapters
00:05
Title
01:01
Agarose Embedding and Vibratome Slicing of Human Lung Tumors
03:51
Immunostaining of Resident CD8 T Cells and Tumor Cells
05:07
Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
07:03
Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
07:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了一种图像驻留肿瘤浸润 CD8 T 细胞标记的人肺肿瘤切片荧光耦合抗体的方法。这项技术允许 real-time 分析 CD8 T 细胞迁移使用共聚焦显微镜。
Tags
Ex Vivo Imaging
Resident CD8 T Lymphocytes
Human Lung Tumor Slices
Confocal Microscopy
Tumor Infiltrating CD8 T Cells
Tumor Immunology
T Cell Migration
Agarose
Vibratome
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