Journal
/
Biology
/
En protokol til brug af gen sæt berigelse analyse til at identificere den passende dyremodel for Translationel forskning
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

En protokol til brug af gen sæt berigelse analyse til at identificere den passende dyremodel for Translationel forskning

DOI:
10.3791/55768-v

•

09:35 min

•

August 16, 2017

•

, , , , ,
1Department of Experimental Toxicology and ZEBET,German Federal Institute for Risk Assessment (BfR), 2Department of Clinical Pharmacology and Toxicology,Charité-Universitätsmedizin Berlin

Chapters

  • 00:05Title
  • 00:54Performing the Game Set Enrichment Analysis (GSEA)
  • 05:46Comparing the GSEA Results and Identifying the Optimal Animal Model
  • 07:55Results: Overlap of Pathway Regulation Identifies Suitable and Unsuitable Animal Models
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

Vi leverer en standardiseret protokol for brug af gen sæt berigelse analyse af transkriptom data til at identificere en ideel musemodel for Translationel forskning.
Denne protokol kan bruges med DNA microarray og RNA sequencing data og kan yderligere udvides til at omfatte andre omik data, hvis der foreligger data.

Tags

Gene Set Enrichment AnalysisAnimal ModelTranslational ResearchGenome DataMouse ModelsInflammatory DiseasesGene Expression StudiesGene Expression DataPhenotype FileMolecular Signature DatabaseDNA Chip AnnotationsGSEA SoftwareGene SymbolsPermutations

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

Computer-Generated Animal Model Stimuli

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

Isolering af ribo Bundet Gryende polypeptider<em> In vitro</em> Til at identificere Translationelle Pause steder langs mRNA

Brug SecM Arrest Sequence som et redskab til at isolere ribo bundne polypeptider

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Xenopus laevis som en model til at identificere Oversættelse Nedskrivning

Komplet Workflow for Analyse af Histon post-translationelle modifikationer Brug Bottom-up-massespektrometri: Fra Histon Extraction til dataanalyse

Read Article