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1Department of Surgery Division of Cardiac Surgery,The Ohio State University Wexner Medical Center, 2The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory,The Ohio State University College of Medicine, 3William G. Lowrie Department of Chemical and Biomolecular Engineering, College of Engineering,The Ohio State University, 4Comprehensive Transplant Center,The Ohio State University Wexner Medical Center
Chapters
- 00:04Introduction
- 00:45Initial Setup
- 02:53Donor Lung Warm Ischemia and Procurement
- 04:14Recipient Rat Preparation and Anastomoses
- 06:12Reperfusion
- 06:35Results: The Wet‐To‐Dry Weight Ratio of The Lobes
- 06:55Conclusion
Here, we present optimizations to a rat lung transplantation model that serve to improve outcomes. We provide a size guide for cuffs based on body weight, a measurement strategy to ascertain the 4th intercostal space, and methods of wound closure and BAL (bronchoalveolar lavage) fluid and tissue collection.
