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JoVE Journal Neuroscience
Quadruple Immunfärbung der olfaktorischen Glühbirne zur Visualisierung von olfaktorischen sensorischen Axon Molecular Identity Codes
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Quadruple Immunfärbung der olfaktorischen Glühbirne zur Visualisierung von olfaktorischen sensorischen Axon Molecular Identity Codes

Article DOI: 10.3791/55893-v • 06:32 min • June 5th, 2017
June 5th, 2017 •
1, 1,2, 1,3
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST)

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Olfaktorische sensorische Neuronen exprimieren eine Vielzahl von Axon-sortierenden Molekülen, um eine korrekte neuronale Schaltung zu etablieren. Dieses Protokoll beschreibt eine immunhistochemische Färbemethode, um kombinatorische Ausdrücke von Axon-sortierenden Molekülen an den Axon-Termini von olfaktorischen sensorischen Neuronen zu visualisieren.

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Neurowissenschaften Ausgabe 124 olfaktorisches System Immunhistochemie Genexpression Axonführung neuronale Schaltungsbildung olfaktorische sensorische Neuronen
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