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Quadruple Immunostaining af Olfactory Bulb til Visualisering af Olfactory Sensory Axon Molecular Identity Codes
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JoVE Journal Neuroscience
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Quadruple Immunostaining af Olfactory Bulb til Visualisering af Olfactory Sensory Axon Molecular Identity Codes

DOI:
10.3791/55893-v

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06:32 min

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June 05, 2017

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, ,
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Chapters

  • 00:05Title
  • 01:11Preparation of Parasagittal Olfactory Bulb Sections
  • 02:33Day 1: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:07Day 2: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:56The Use of Fluorescence Intensity Measurements
  • 04:26Results: Quantification of Axon-sorting Molecules in the Olfactory Bulb
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

Olfaktoriske sensoriske neuroner udtrykker et bredt udvalg af axon-sorteringsmolekyler for at etablere et passende neuralt kredsløb. Denne protokol beskriver en immunhistokemisk farvningsfremgangsmåde til visualisering af kombinatoriske udtryk for axon-sorteringsmolekyler ved axon termini af olfaktoriske sensoriske neuroner.

Tags

Quadruple ImmunostainingOlfactory BulbOlfactory Sensory AxonMolecular Identity CodesAxon Sorting MoleculesOlfactory DevelopmentAxon GuidanceSynapse FormationOlfactory Sensory Neuron RegenerationCryostat SectioningImmunostainingPrimary AntibodiesSecondary Antibodies

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