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嗅球的四次免疫染色用于嗅觉感觉轴突分子识别码的可视化
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JoVE Journal Neuroscience
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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嗅球的四次免疫染色用于嗅觉感觉轴突分子识别码的可视化

DOI:
10.3791/55893-v

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06:32 min

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June 05, 2017

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, ,
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Chapters

  • 00:05Title
  • 01:11Preparation of Parasagittal Olfactory Bulb Sections
  • 02:33Day 1: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:07Day 2: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:56The Use of Fluorescence Intensity Measurements
  • 04:26Results: Quantification of Axon-sorting Molecules in the Olfactory Bulb
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

嗅觉神经元表达各种各样的轴突分选分子，建立适当的神经电路。该方案描述了一种免疫组织化学染色方法来观察嗅觉神经元轴突末端的轴突分选分子的组合表达。

Tags

Quadruple ImmunostainingOlfactory BulbOlfactory Sensory AxonMolecular Identity CodesAxon Sorting MoleculesOlfactory DevelopmentAxon GuidanceSynapse FormationOlfactory Sensory Neuron RegenerationCryostat SectioningImmunostainingPrimary AntibodiesSecondary Antibodies

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