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1Department of Medicine, Institute of Translational Pharmacology,Italian National Research Council, 2Epigenetics and Regenerative Medicine,IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division,King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences,University of Modena and Reggio Emilia
Chapters
- 00:05Title
- 01:00Muscle Injury and Tissue Harvest
- 03:12Cryosectioning, Immunostaining, and Confocal Microscopy Imaging
- 06:47Results: Representative Injured Muscle Tissue and Muscle Satellite Cell (MuSC) Analyses
- 07:43Conclusion
L'autophagie active est associée à la régénération musculaire productive, ce qui est essentiel pour l'activation des cellules souches musculaires (MuSC). Ici, nous fournissons un protocole pour la détection in situ de LC3, un marqueur autophagique dans les muSCs MyoD-positives de sections de tissu musculaire à partir de souris témoins et blessées.
