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1Department of Medicine, Institute of Translational Pharmacology,Italian National Research Council, 2Epigenetics and Regenerative Medicine,IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division,King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences,University of Modena and Reggio Emilia
Chapters
- 00:05Title
- 01:00Muscle Injury and Tissue Harvest
- 03:12Cryosectioning, Immunostaining, and Confocal Microscopy Imaging
- 06:47Results: Representative Injured Muscle Tissue and Muscle Satellite Cell (MuSC) Analyses
- 07:43Conclusion
Aktiv autofagi er assosiert med produktiv muskelregenerasjon, noe som er viktig for aktivering av muskelstamceller (MuSC). Her gir vi en protokoll for in situ- deteksjon av LC3, en autophagy markør i MyoD-positive MuSCs av muskelvevseksjoner fra kontroll og skadede mus.
