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1Department of Medicine, Institute of Translational Pharmacology,Italian National Research Council, 2Epigenetics and Regenerative Medicine,IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division,King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences,University of Modena and Reggio Emilia
Chapters
- 00:05Title
- 01:00Muscle Injury and Tissue Harvest
- 03:12Cryosectioning, Immunostaining, and Confocal Microscopy Imaging
- 06:47Results: Representative Injured Muscle Tissue and Muscle Satellite Cell (MuSC) Analyses
- 07:43Conclusion
La autofagia activa se asocia con la regeneración muscular productiva, que es esencial para la activación de las células madre musculares (MuSC). Aquí, proporcionamos un protocolo para la detección in situ de LC3, un marcador de autofagia en MyoD-positivos MuSCs de secciones de tejido muscular de control y los ratones lesionados.
