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Immunology and Infection
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纯化膜隔间跨演示文稿中的外源抗原内质网关联降解
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
Please note that all translations are automatically generated.
Click here for the English version.
纯化膜隔间跨演示文稿中的外源抗原内质网关联降解
DOI:
10.3791/55949-v
•
12:48 min
•
August 21, 2017
•
Jun Imai
,
Mayu Otani
,
Takahiro Sakai
,
Shinichi Hatta
1
Laboratory of Physiological Chemistry, Faculty of Pharmacy
,
Takasaki University of Health and Welfare
Chapters
00:05
Title
01:13
Preparation of Microsomes
04:44
Purification of Microsomes with bOVA Underground ERAD
06:59
In Vitro
Reconstruction of ERAD Ubiquination of bOVA Using Purified Microsomes
09:04
Results: Purified Microsomes and
In Vitro
Ubiquination of the Exogenous Antigen
11:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的方法是一种新的囊泡隔离协议,允许纯化细胞的车厢外源性抗原处理由内质网关联退化跨演示文稿中的地方。
Tags
Membrane Compartment
Endoplasmic Reticulum-associated Degradation (ERAD)
Cross-presentation
Exogenous Antigen
Dendritic Cells
Biotinylated Ovalbumin (bOVA)
Vesicle Isolation
Microsome
Ubiquitination
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