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Cancer Research

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JoVE Journal Cancer Research
Natural Killer (NK) og CAR-NK Cell Expansion Method ved bruk av Membran Bound-IL-21-Modified B Cell Line
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Natural Killer (NK) og CAR-NK Cell Expansion Method ved bruk av Membran Bound-IL-21-Modified B Cell Line

Article DOI: 10.3791/62336-v • 11:02 min • February 8th, 2022
February 8th, 2022 •
1,2, 1, 1, 1,3
1Department of Pathology, Immunology and Laboratory Medicine, Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School, Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School, Rutgers-The State University of New Jersey

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Her presenterer vi en metode for å utvide perifert blod naturlig morder (PBNK), NK-celler fra levervev og kimær antigenreseptor (CAR) -NK-celler avledet fra perifere blodmononukleære celler (PBMC) eller ledningsblod (CB). Denne protokollen demonstrerer utvidelsen av NK- og CAR-NK-celler ved bruk av 221 mIL-21 materceller i tillegg til den optimaliserte renheten til utvidede NK-celler.

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