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JoVE Journal Cancer Research
Evaluering av celledød ved hjelp av cellefri supernatant av probiotika i tredimensjonale sfæroidkulturer av kolorektale kreftceller
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Evaluering av celledød ved hjelp av cellefri supernatant av probiotika i tredimensjonale sfæroidkulturer av kolorektale kreftceller

Article DOI: 10.3791/61285-v • 06:07 min • June 13th, 2020
June 13th, 2020 •
*1,2, *3, 1,4,5, 1, 1
1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST)
* These authors contributed equally

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Her presenteres metoder for å forstå antikrefteffekter av Lactobacillus cellefri supernatant (LCFS). Kolorektal kreftcellelinjer viser celledødsfall når de behandles med LCFS i 3D-kulturer. Prosessen med å generere sfæroider kan optimaliseres avhengig av stillaset, og analysemetodene som presenteres er nyttige for å evaluere de involverte signalveiene.

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Kreftforskning Utgave 160 probiotika Lactobacillus fermentum kolorektal kreft sfæroid 3D-kultur Lactobacillus cellefri supernatant (LCFS)
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