Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Producción de hámsters sirios dorados…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Producción de hámsters sirios dorados genéticamente por inyección Pronuclear del complejo CRISPR/Cas9
Evaluating Primary Blast Effects In Vitro
Journal (Bioengineering)
Evaluating Primary Blast Effects In Vitro
Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood
Journal (Bioengineering)
Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood
 
Click here for the English version

Producción de hámsters sirios dorados genéticamente por inyección Pronuclear del complejo CRISPR/Cas9

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Inyección pronuclear de (PN) del cluster regularmente otro corto repite palindrómico (CRISPR) y sistema CRISPR asociados 9 proteína nucleasa (CRISPR/Cas9) es un método altamente eficiente para la producción de transgénicos hámsters sirios dorados. Adjunto, describimos el protocolo detallado de la inyección del PN para la producción de hamsters de gene knockout con el sistema CRISPR/Cas9.

Tags

Bioingeniería número 131 inyección Pronuclear CRISPR/Cas9 cigoto edición knockout gene transferencia de embriones modelo animal Hámster Sirio dorado
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter