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Journal / Bioengineering / Produksjon av genmodifiserte Golden syrer…
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Bioengineering

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JoVE Journal Bioengineering
Produksjon av genmodifiserte Golden syrer Hamsters ved Pronuclear injeksjon av CRISPR/Cas9 komplekset
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Produksjon av genmodifiserte Golden syrer Hamsters ved Pronuclear injeksjon av CRISPR/Cas9 komplekset

Article DOI: 10.3791/56263-v • 06:12 min • January 9th, 2018
January 9th, 2018 •
*1, *1,2, 1, 3, 3,4, 2,5, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
* These authors contributed equally

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Pronuclear (PN) injeksjon av de klynget regelmessig interspaced kort palindromic gjentar (CRISPR) og CRISPR-assosiert protein-9 nuclease (CRISPR/Cas9) er en svært effektiv metode for å produsere genmodifiserte golden syrer hamsters. Her beskriver vi detaljerte PN injeksjon protokollen for produksjon av genet knockout hamstere med CRISPR/Cas9-systemet.

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Bioteknologi problemet 131 Pronuclear injeksjon CRISPR/Cas9 gene redigering knockout zygote embryoer dyremodell golden Syrer hamster
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