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Journal
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Biochemistry
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碳水化合物转运基质结合蛋白 SP0092 的生化和结构表征
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Please note that all translations are automatically generated.
Click here for the English version.
碳水化合物转运基质结合蛋白 SP0092 的生化和结构表征
DOI:
10.3791/56294-v
•
08:53 min
•
October 02, 2017
•
Simone Culurgioni
2
,
Minzhe Tang
2
,
David R. Hall
,
Martin A. Walsh
2
1
Diamond Light Source
,
Harwell Science & Innovation Campus
,
2
Research Complex at Harwell
,
Harwell Science & Innovation Campus
Chapters
00:05
Title
00:42
Buffer and Substrate-binding Protein (SBP) Preparation
02:50
SBP Crystallization and Data Collection
06:19
Results: Biochemical and Structural Characterization of SP0092
08:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
一个简化的协议, 以执行广泛的生物化学和结构表征的碳水化合物基质结合蛋白从
肺炎链球菌
。
Tags
Carbohydrate Transport
Substrate-binding Protein
Streptococcus Pneumoniae
Biochemical Characterization
Structural Characterization
Buffer Stability
Oligomerization
Crystallization
Size-exclusion Chromatography
Multi-angle Light Scattering
Pre-crystallization Testing
Protein Concentration
Crystallization Screening
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