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Journal / Immunology and Infection / Målretting cystein Thiols for in Vitro…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Målretting cystein Thiols for in Vitro områdespesifikke glykosylering rekombinante proteiner
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Målretting cystein Thiols for in Vitro områdespesifikke glykosylering rekombinante proteiner

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Biokjemiske og strukturelle analyser av glycosylated proteiner krever relativt store mengder homogen prøver. Her presenterer vi en effektiv kjemisk metode for områdespesifikke glykosylering rekombinante proteiner renset fra bakterier av målretting reaktive Cys thiols.

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Immunologi problemet 128 glykosylering methanethiosulfonate cystein thiol endring stromal samhandling molekyl-1 EFSAM løsning kjernefysiske magnetisk resonans spektroskopi massespektrometri
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