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December 20, 2017
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Dette er en protokoll for å studere intracellulær protein-protein interaksjoner basert på biotin-avidin rullegardinmenyen system med nyheten av kombinerer celle-gjennomtrengende sekvenser. Hovedfordelen er at målet sekvensen er ruges med levende celler i stedet for cellen lysates og derfor samspillet skjer innen mobilnettet sammenheng.
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Cite this Article
Jaraíz-Rodríguez, M., González-Sánchez, A., García-Vicente, L., Medina, J. M., Tabernero, A. Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions. J. Vis. Exp. (130), e56457, doi:10.3791/56457 (2017).
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