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成年大鼠心肌细胞的分离与培养
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JoVE Journal Medicine
Isolation and Cultivation of Adult Rat Cardiomyocytes
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成年大鼠心肌细胞的分离与培养

DOI:
10.3791/56634-v

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11:06 min

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October 19, 2017

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, ,
1Institute of Physiology,Justus Liebig University Giessen

Chapters

  • 00:05Title
  • 01:15Preparation of Langendorff Perfusion System
  • 03:09Extraction and Isolation of the Heart
  • 08:21Results: The Appearance and Behavior of Adult Rat Ventricular Cardiomyocytes (ARVC) during Six Days of Cultivation
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个分离和培养成年大鼠心室心肌细胞 (ARVC) 的协议。隔离 ARVC 可用于短期和长期种植。ARVC 的分离和培养可在开发新的心脏疾病治疗方案中发挥关键作用。

Tags

Keywords: Adult Rat CardiomyocytesCardiac BiologyCardiac PathophysiologyCardiac DedifferentiationCardiac HypertensionHeart FailureLangendorff Perfusion SystemCollagenasePowell MediumWistar Rats

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