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Luciferase Complementation Imaging analysen i Nicotiana benthamiana blader for Transiently bestemme Protein-protein samhandling Dynamics
JoVE Journal
Biology
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JoVE Journal Biology
Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
DOI:

07:55 min

November 20, 2017

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Chapters

  • 00:05Title
  • 00:42Preparation of Plants
  • 02:02Transient Expression in N. benthamiana Leaves
  • 04:49Detecting Luciferase Activity
  • 06:10Results: Representative Transient Luciferase Complementation Assay Determining COP1-SPA1 Interaction Dynamics
  • 07:00Conclusion

Summary

Automatic Translation

Dette manuskriptet beskriver en enkel og rask eksperimentelle prosedyren for å bestemme protein-protein interaksjoner basert på måling av luciferase aktivitet.

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