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Luciferase Complementation Imaging analysen i Nicotiana benthamiana blader for Transiently bestemme Protein-protein samhandling Dynamics
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Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Luciferase Complementation Imaging analysen i Nicotiana benthamiana blader for Transiently bestemme Protein-protein samhandling Dynamics

DOI:
10.3791/56641-v

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07:55 min

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November 20, 2017

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, ,
1Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences,Nanjing Normal University

Chapters

  • 00:05Title
  • 00:42Preparation of Plants
  • 02:02Transient Expression in N. benthamiana Leaves
  • 04:49Detecting Luciferase Activity
  • 06:10Results: Representative Transient Luciferase Complementation Assay Determining COP1-SPA1 Interaction Dynamics
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskriptet beskriver en enkel og rask eksperimentelle prosedyren for å bestemme protein-protein interaksjoner basert på måling av luciferase aktivitet.

Tags

Luciferase Complementation ImagingProtein-protein InteractionTransient ExpressionLuminometerCCD CameraSodium HypochloriteNicotiana BenthamianaAgrobacterium TumefaciensPlasmidLB MediumKanamycinRifampicin

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