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Undersøge de skadelige effekter af lav pres Plasma sterilisation på overlevelse af Bacillus subtilis sporer ved hjælp af Live celle mikroskopi
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Undersøge de skadelige effekter af lav pres Plasma sterilisation på overlevelse af Bacillus subtilis sporer ved hjælp af Live celle mikroskopi

Article DOI: 10.3791/56666-v • 10:03 min • November 30th, 2017
November 30th, 2017 •
1, 1,2,3, 2, 4, 2, 4, 3, 1
1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

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Denne protokol illustrerer de vigtige træk skridt forpligtet til at vurdere relevansen af overvågning vitalitet parameter og DNA reparationsprocesser i genoplive Bacillus subtilis sporer efter behandling med lavt tryk plasma med tracking Fluorescens-mærket DNA reparere proteiner via tidsopløst Fluorescens mikroskopi og scanning Elektron Mikroskopi.

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Cellebiologi sag 129 Plasma sterilisation dekontaminering Bacillus subtilis spore modstand DNA reparation levende celle imaging SEM cLSM Fluorescens mikroskopi
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