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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
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Chapters
- 00:04Title
- 00:48Preparing the NIOSH Aerosol Sampler
- 02:00Personal Aerosol Sampling
- 02:47Extraction of Genomic DNA from Air Samples
- 07:46Amplification of Fungal Ribosomal DNA
- 09:37Verification of Fungal ITS Amplification Using Agarose Gel Electrophoresis
- 10:12Results: Sequence-based Approach to Analyze Fungal Diversity in an Occupational Environment Using Bioaerosols
- 11:31Conclusion
Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment methods.
