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Två-foton kalcium Imaging i nervcellernas dendriter i hjärnan skivor
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
DOI:

10:35 min

March 15, 2018

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Chapters

  • 00:04Title
  • 00:46Hippocampal Slice Preparation
  • 02:03Whole-cell Patch-clamp Recordings
  • 04:33Two-photon Ca2+ Imaging
  • 08:36Results: Postsynaptic Ca2+ Transients Evoked by a Burst of Electrical Stimulation
  • 09:49Conclusion

Summary

Automatic Translation

Vi presenterar en metod som kombinerar hela-cell patch-clamp inspelningar och två-photon imaging för att spela in Ca2 + transienter i nervcellernas dendriter i akut hjärnskada skivor.

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