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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Chemistry
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JoVE Journal Chemistry
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

DOI:
10.3791/56814-v

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09:33 min

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March 21, 2018

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, , , , ,
1Department of Chemistry,Capital Normal University, 2College of Life Sciences,Capital Normal University

Chapters

  • 00:04Title
  • 00:50Small-scale λ Exonuclease Reaction
  • 02:40Relative Affinity and Specificity Test by Competitive Assays
  • 04:17Fabrication and Electrochemical Measurements of Diethylhexyl Phthalate (DEHP) Electrochemical Biosensors
  • 07:52Results: Optimization of λ Exonuclease Reaction and Electrochemical Aptasensor Evaluation
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the in vitro selection and characterization of group-specific phthalic acid ester- binding DNA aptamers is presented. The application of the selected aptamer in an electrochemical aptasensor is also included.

Tags

Keywords: Phthalic Acid EsterDNA AptamerElectrochemical AptasensorHydrophobic MoleculesGroup-specific AptamersLambda ExonucleaseNative PAGE GelMagnetic BeadsPAE Binding Buffer

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