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An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
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Chemistry
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JoVE Journal Chemistry
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

DOI:
10.3791/56858-v

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08:34 min

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December 18, 2017

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, ,
1Centre for Epigenetics and Disease Prevention, Department of Molecular and Cellular Medicine, Institute of Biosciences and Technology, College of Medicine,Texas A&M University, 2Centre for Translational Cancer Research, Department of Medical Physiology, Institute of Biosciences and Technology, College of Medicine,Texas A&M University

Chapters

  • 00:05Title
  • 00:48Cell Culture, Plasmid Transfection, and Chemical Induction
  • 01:45Quantification of Rapamycin-indiced 5-hydroxymethylcystosine (5hmc) Production by Immunostaining
  • 03:08Dot-blot Assay Quantify 5hmc and 5-methylcystosine (5mc) Amounts
  • 07:03Results: Validation of Chemically Induced 5hmc Levels
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

Detailed protocols for introducing an engineered split-TET2 enzyme (CiDER) into mammalian cells for chemical inducible DNA hydroxymethylation and epigenetic remodeling are presented.

Tags

Keywords: Split-TET2CiDERDNA HydroxymethylationEpigenetic RemodelingChemical InductionRapamycinFlow Cytometry5-hydroxymethylcytosine (5hmC)Genomic DNASlot Blot Analysis

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