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Funktionel billeddannelse af Viral transskription fabrikker ved hjælp af 3D Fluorescens mikroskopi
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Funktionel billeddannelse af Viral transskription fabrikker ved hjælp af 3D Fluorescens mikroskopi

Article DOI: 10.3791/56832-v • 09:03 min • January 18th, 2018
January 18th, 2018 •
1, 2, 1
1Department of Dermatology, University of California Davis School of Medicine, University of California, Davis, 2Center for Biophotonics, Department of Biochemistry and Molecular Medicine, University of California, Davis

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Viral transcriptional fabrikker er diskrete strukturer, der er beriget med cellulære RNA polymerase II at øge virale gen transskription under genaktivering. Her, er en metode til at finde websteder af aktivt transskribere viral kromatin i 3D nukleare plads ved en kombination af immunofluorescens farvning og i situ RNA hybridisering beskrevet.

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Mikrobiologi sag 131 Viral transskription factory KSHV Kaposis sarkom-associerede Herpesvirus transskription RNA Polymerase II RNA-fisk aktive transskription
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