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Journal / Developmental Biology / Udvikling af et In Vitro Assay at kvantificere…
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Developmental Biology

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JoVE Journal Developmental Biology
Udvikling af et In Vitro Assay at kvantificere hæmatopoietisk stilk og stamfader celler (HSPCs) i at udvikle zebrafisk embryoner
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Udvikling af et In Vitro Assay at kvantificere hæmatopoietisk stilk og stamfader celler (HSPCs) i at udvikle zebrafisk embryoner

Article DOI: 10.3791/56836-v • 10:28 min • November 30th, 2017
November 30th, 2017 •
1, 1
1Department of Biological Sciences, California State University, Chico

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Her præsenterer vi en enkel metode til at kvantificere hæmatopoietisk stilk og stamfader celler (HSPCs) i embryonale zebrafisk. HSPCs fra dissocierede zebrafisk er belagt i methylcellulose med understøttende faktorer, differentiere til modne blod. Dette giver mulighed for påvisning af blod pixeldefekter og giver mulighed for narkotika screening for at foretages nemt.

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Udviklingsmæssige biologi sag 129 zebrafisk bloddannelsen hæmatopoietisk stilk og stamfader celler (HSPCs) udvikling in vitro- methylcellulose klonede assay
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