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定量造血干和祖细胞 (HSPCs) 在斑马鱼胚胎发育中的体外试验研究

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JoVE Journal Developmental Biology

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

Please note that all translations are automatically generated. Click here for the English version.

定量造血干和祖细胞 (HSPCs) 在斑马鱼胚胎发育中的体外试验研究

DOI:

10.3791/56836-v

•

10:28 min

•

November 30, 2017

•

A.C. Berrun, D.L. Stachura

¹Department of Biological Sciences,California State University, Chico

Chapters

00:05Title
01:05Bleach and Dechorionate 48 hpf Zebrafish Embryos
03:19Embryos for Dissociation
05:15Preparation of Dissociated Embryos
06:21Preparation of Methylcellulose
07:30Addition of Dissociated Embryos to Methylcellulose
08:53Results: Quantitation of Zebrafish Embryonic Hematopoietic Stem and Progenitor Cells
09:32Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个简单的方法, 定量造血干细胞和祖细胞 (HSPCs) 在胚胎斑马鱼。HSPCs 从分离的斑马鱼被镀在纤维素与支持因素, 分化成成熟的血液。这使得检测血液缺陷和允许药物筛选很容易进行。

Tags

Keywords: In Vitro Assay Hematopoietic Stem Cells Hematopoietic Progenitor Cells Zebrafish Embryos Blood Progenitor Formation Gene Knockdown Morphants Bleaching Dechorionation E3 Medium

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