Journal
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Cuantificación de endógeno auxina y citoquinina durante cultura de entrenudo de Ipecacuana
JoVE Journal
Environment
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JoVE Journal Environment
Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
DOI:

07:57 min

March 15, 2018

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Chapters

  • 00:05Title
  • 00:51Growth Conditions to Induce Adventitious Shoots of Ipecac
  • 01:46Extraction and Purification of Phytohormones
  • 04:46LC-MS/MS Analysis of IAA and CKs
  • 05:41Results: IAA and CK Levels in Adventitious Shoots Change During Five Weeks of Culture
  • 07:12Conclusion

Summary

Automatic Translation

Brotes adventicios pueden ser inducidas en los segmentos internodales de Ipecacuana sin tratamiento de fitohormonas. Para evaluar la dinámica de la fitohormona durante la formación de brotes adventicios, medimos endógeno auxina y citocinina en segmentos internodales por LC-MS/MS.

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