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Journal / Neuroscience / 密度梯度离心法分离小鼠原发胶质细胞的研究…
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JoVE Journal Neuroscience
密度梯度离心法分离小鼠原发胶质细胞的研究
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密度梯度离心法分离小鼠原发胶质细胞的研究

Article DOI: 10.3791/57065-v • 10:22 min • February 16th, 2018
February 16th, 2018 •
*1, 1,2, 1, *1
1The Ritchie Centre, Hudson Institute of Medical Research, 2Department of Obstetrics & Gynaecology, Monash University
* These authors contributed equally

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本文提出了一种从小鼠脑中分离原发胶质细胞的方法。这种技术有助于促进目前对神经系统状况的理解。将密度梯度离心和磁分离相结合, 产生高纯样品的充分屈服。此外, 我们概述了小胶质细胞的表征步骤。

Tags

神经科学 问题 132 小胶质细胞 分离 原发体细胞 细胞培养 炎症 动物模型 老鼠 新生儿 神经科学 围产期脑损伤
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