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密度梯度离心法分离小鼠原发胶质细胞的研究

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

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密度梯度离心法分离小鼠原发胶质细胞的研究

DOI:

10.3791/57065-v

•

10:21 min

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February 16, 2018

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Jessica C. Stark*¹, Euan Wallace², Rebecca Lim, Bryan Leaw*¹

¹The Ritchie Centre,Hudson Institute of Medical Research, ²Department of Obstetrics & Gynaecology,Monash University

Chapters

00:04Title
00:36Enzymatic Digestion
02:52Myelin Debris Removal
05:53Magnetic Activated Cell Sorting
08:01Results: Analysis of Microglia Survival After Inflammatory Insult
08:42Conclusion

Summary

Automatic Translation

本文提出了一种从小鼠脑中分离原发胶质细胞的方法。这种技术有助于促进目前对神经系统状况的理解。将密度梯度离心和磁分离相结合, 产生高纯样品的充分屈服。此外, 我们概述了小胶质细胞的表征步骤。

Tags

Microglia Isolation Density Gradient Centrifugation Primary Microglia Neuroinflammation Immunomodulation Drug Screening Brain Dissection Cell Straining Cell Viability

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