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通过磁活化细胞分选在脱髓鞘动物模型中分离小鼠原代小胶质细胞
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
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通过磁活化细胞分选在脱髓鞘动物模型中分离小鼠原代小胶质细胞

DOI:
10.3791/63511-v

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04:53 min

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April 05, 2022

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, , , ,
1Department of Neurology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology

Chapters

  • 00:04Introduction
  • 00:42Mice Dissection and Tissue Dissociation
  • 01:35Debris Removal and Magnetic Separation of Microglial Cells
  • 03:26Results: Gating Strategy Used in Flow Cytometry Analysis Before and After MACS and Gating Strategy for Living/Dead Single Cells After MACS
  • 04:27Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一种方案，利用柱状磁激活细胞分选在脱髓鞘疾病的动物模型中分离和纯化原代小胶质细胞。

Tags

Mouse Primary MicrogliaMagnetic-activated Cell SortingDemyelinationCorpus CallosumEnzyme DigestionCD11b BeadsPositive SelectionMagnetic Field

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